Yunnan University 1 article published in JoVE Neuroscience Organotypic Retinal Explant Cultures from Macaque Monkey Wenrong Xu1, Yujie Dong1, Yan Li1, Zhulin Hu1, François Paquet-Durand2, Kangwei Jiao1 1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.