Xi'an Institute of Tissue Engineering and Regenerative Medicine 2 articles published in JoVE Biology Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues Yuan Cao*1,2, Ji-Yu Qiu*1,3, Da Chen1,2, Chen-Yao Li1,4, Shu-Juan Xing1,5, Chen-Xi Zheng1, Xin Liu2, Yan Jin1,6, Bing-Dong Sui1 1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6 The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos. Biology Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells Shu-Juan Xing1,2, Kai-Chao Zhang2,3, Si-Yuan Tang2,4, Lu Liu2,5, Yuan Cao2,5, Chen-Xi Zheng2, Bing-Dong Sui2, Yan Jin2,3 1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3 The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.
