Capital Medical University, China Rehabilitation Research Center 1 article published in JoVE Immunology and Infection Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique Linlin Zhang*1,2, Meng Zhang*1,2,3, Mengjia Liu1,2, Junhong Ai1,2, Jiao Tian1,2, Huizheng Ge4, Ran Wang1,2, Zhengde Xie1,2 1 The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies.
