Key Laboratory of Traditional Chinese Medicine Regimen and Health of Sichuan Province 1 article published in JoVE Medicine Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia Baihan Zeng*1,2,3, Luming Qi*1,2,3, Sha Wu*1,2,3, Nannan Liu1,2,3, Jie Wang1,2,3, Kaidi Nie1,2,3, Lina Xia1,2,3, Shuguang Yu2,3 1School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, 2State Administration of Traditional Chinese Medicine Key Laboratory of Traditional Chinese Medicine, Regimen and Health, Chengdu University of Traditional Chinese Medicine, 3Key Laboratory of Traditional Chinese Medicine Regimen and Health of Sichuan Province The present protocol describes an integrated strategy for exploring the key targets and mechanisms of Fructus Phyllanthi against hyperlipidemia based on network pharmacology prediction and metabolomics verification.