Zhejiang University School of Medicine, National Clinical Research Center for Child Health 1 article published in JoVE Developmental Biology Generating Kidney Organoids in Suspension from Induced Pluripotent Stem Cells Langping Gao*1,2, Yue Wang*3, Gang Wang4, Hangdi Wu2, Qingtao Yan1, Jingjing Wang1, Fei Liu1, Haidong Fu1, Wei Li5, Lidan Hu1, Jianhua Mao1,2 1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health This protocol presents a comprehensive and efficient method for producing kidney organoids from induced pluripotent stem cells (iPSCs) using suspension culture conditions. The primary emphasis of this study lies in the determination of the initial cell density and the WNT agonist concentration, thereby benefiting investigators interested in kidney organoid research.
