The First Affiliated Hospital of Kunming Medical University 2 articles published in JoVE Neuroscience Harvest and Primary Culture of Leptomeningeal Lymphatic Endothelial Cells Hong-Ji Deng1, Kun Wu2, Han-Fu Yu1, Yong-Jin Zhang1,3, Yun-Cong Li1, Chong Li1, Fei Wang1 1Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, 2Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, 3Clinical Medical Research Center, The First Affiliated Hospital of Kunming Medical University Leptomeningeal lymphatic endothelial cells (LLECs), a recently identified intracranial cell type, have poorly understood functions. This study presents a reproducible protocol for harvesting LLECs from mice and establishing in vitro primary cultures. This protocol is designed to enable researchers to delve into the cellular functions and potential clinical implications of LLECs. Neuroscience Imaging Vital and Non-vital Brain Pericytes in Brain Slices following Subarachnoid Hemorrhage Yong-Jin Zhang1,2, Yun-Cong Li1, Han-Fu Yu1, Chong Li1, Hong-Ji Deng1, Yue-Hong Dong2, Gui-Bo Li1, Fei Wang1 1Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, 2Clinical Medical Research Center, The First Affiliated Hospital of Kunming Medical University The preliminary inquiry confirms that subarachnoid hemorrhage (SAH) causes brain pericyte demise. Evaluating pericyte contractility post-SAH requires differentiation between viable and non-viable brain pericytes. Hence, a procedure has been developed to label viable and non-viable brain pericytes concurrently in brain sections, facilitating observation using a high-resolution confocal microscope.
