Era University 1 article published in JoVE Biology Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo Neha Kumari1, Santosh Kumar Yadav*1, Ravi Prakash*1, Abu Junaid Siddiqui1, Mohsin Ali Khan2, Syed Shadab Raza1,3 1Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College Hospital, Era University, 2Era University, 3Department of Stem Cell Biology and Regenerative Medicine, Era University This paper describes ischemia-reperfusion (I/R) modeling in a 3-day chick embryo using a spinal needle customized hook to better understand I/R development and treatment. This model is simple, quick, and inexpensive.