Christian Medical College 1 article published in JoVE Cancer Research Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype Saswati Das1,2, Raveen Stephen Stallon Illangeswaran1,2, Smitha Ijee2,3, Sanjay Kumar2,3, Shaji Ramachandran Velayudhan1,2,3, Poonkuzhali Balasubramanian1,2,3 1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).