Indian Institute of Technology-Kanpur 1 article published in JoVE Bioengineering Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint Aman Mahajan*1,2, Sugata Hazra*1,2, Aditya Arora1,3, Dhirendra S. Katti1,2 1Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, 2The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology-Kanpur, 3Mechanobiology Institute, National University of Singapore Infrapatellar fat pad mesenchymal stem cells (IFP-MSCs) can be isolated easily from the infrapatellar fat pad of the knee joint. They proliferate well in vitro, form CFU-F colonies, and differentiate into adipogenic, chondrogenic, and osteogenic lineages. Herein, the methodology for the isolation, expansion, and differentiation of IFP-MSCs from goat stifle joint is provided.
