University of Jordan 3 articles published in JoVE Medicine Quantification of Optic Nerve Cross Sectional Area on MRI: A Novel Protocol using Fiji Software Nosaiba Al-Ryalat1, Saif Aldeen AlRyalat2, Lna Malkawi1, Muayad Azzam3, Sana Mohsen2 1Department of Radiology and Nuclear Medicine, The University of Jordan, 2Department of Special Surgery, The University of Jordan, 3Medical student, School of medicine, The University of Jordan We provided a detailed protocol for a standardized method of optic nerve assessment and quantification using MRI, utilizing a widely available imaging sequence, and open access software for image analysis. Following this standardized protocol would provide meaningful data for comparison between different patients and different studies. Biology Bovine Ovarian Cortex Tissue Culture Courtney M. Sutton*1, Shelby A. Springman*1, Mohamed A. Abedal-Majed2, Andrea S. Cupp1 1Department of Animal Science, University of Nebraska-Lincoln, 2Department of Animal Production, School of Agriculture, University of Jordan In vitro culture of bovine ovarian cortex and the effect of nutritional Stair-step diet on ovarian microenvironment is presented. Ovarian cortex pieces were cultured for seven days and steroids, cytokines, and follicle stages were evaluated. The Stair-Step diet treatment had increased steroidogenesis resulting in follicle progression in culture. Medicine Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases Saif Aldeen S. AlRyalat1, Lna W. Malkawi2, Shaher M. Momani3,4 1Department of Ophthalmology, University of Jordan, 2Department of Radiology and Nuclear Medicine, University of Jordan, 3Dean of Scientific Research, University of Jordan, 4Nonlinear Analysis and Applied Mathematics (NAAM) Research Group, Faculty of Science, King Abdulaziz University Literature databases are commonly used to assess publications in a certain subject, discipline, country, or region of the world, a practice known as bibliometric analysis. The current protocol details how to use PubMed, Scopus, and Web of Science databases to do bibliometric analysis.