National Agriculture and Food Research Organization 2 articles published in JoVE Genetics Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains Kaori Nishimura*1, Miho Asaoka*2, Yurina Sakamaki*2, Tatsuya Fukumoto*3,4, Daisuke Tanaka3, Satoru Kobayashi2, Toshiyuki Takano-Shimizu-Kouno1 1KYOTO Drosophila Stock Center, Kyoto Institute of Technology, 2Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 3Research Center of Genetic Resources, National Agriculture and Food Research Organization, 4Shizuoka Prefectural Ogasa High School A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos. Environment Tracking Infiltration Front Depth Using Time-lapse Multi-offset Gathers Collected with Array Antenna Ground Penetrating Radar Hirotaka Saito1, Seiichiro Kuroda2, Toshiki Iwasaki1, Haruyuki Fujimaki3, Nobuhito Nagai4, Jacopo Sala5 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 2National Institute for Rural Engineering, National Agriculture and Food Research Organization, 3Arid Land Research Center, Tottori University, 4GeoFive Co. Ltd, 53D-Radar Here we present a Ground Penetrating Radar (GPR) system based on a ground-coupled, densely populated antenna array for monitoring the dynamic process of subsurface water infiltration. A time-lapse radar image of the infiltration process allowed estimating the depth of the wetting front during the course of the infiltration process.
