Aichi Medical University 2 articles published in JoVE Immunology and Infection Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA Md. Monirul Islam1, Umme Salma2, Takayuki Irahara2, Eizo Watanabe2, Naoshi Takeyama2 1Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine, Aichi Medical University We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils. Developmental Biology Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary Kouji Komatsu1, Akira Iwase2,3, Tomohiko Murase2, Satoru Masubuchi1 1Department of Physiology, Aichi Medical University, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Maternal and Perinatal Medicine, Nagoya University Hospital Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.