Tohoku University Graduate School of Dentistry 2 articles published in JoVE Biology Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes Aseel Marahleh1, Hideki Kitaura1, Saika Ogawa1, Wei-Ren Shen1, Jiawei Qi1, Fumitoshi Ohori1, Takahiro Noguchi1, Yasuhiko Nara1, Adya Pramusita1, Ria Kinjo1, Itaru Mizoguchi1 1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS). Immunology and Infection Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro Aseel Marahleh1, Hideki Kitaura1, Masahiko Ishida1, Kazuhiro Shima1, Akiko Kishikawa1, Saika Ogawa1, Wei-Ren Shen1, Jiawei Qi1, Fumitoshi Ohori1, Takahiro Noguchi1, Yasuhiko Nara1, Itaru Mizoguchi1 1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry This protocol includes dissection of murine long bones and bone marrow isolation, to generate bone marrow macrophages and produce osteoclasts using macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor Kappa-B ligand (RANKL) or tumor necrosis factor alpha (TNF-α) and their arrest by anti-c-fms antibody, M-CSF receptor.
