JAIST Venture Business Laboratory, Ishikawa Create Labo 1 article published in JoVE Bioengineering A Nonsequencing Approach for the Rapid Detection of RNA Editing Ruchika 1, Toshifumi Tshukahara1, Manish Biyani1,2 1Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, 2BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
