Seoul National University Hospital 7 articles published in JoVE Bioengineering Design and Synthesis of a Reconfigurable DNA Accordion Rack Yeongjae Choi*1, Hansol Choi*1, Amos C. Lee*2, Sunghoon Kwon1,2,3,4 1Department of Electrical and Computer Engineering, Seoul National University, 2Interdisciplinary Program for Bioengineering, Seoul National University, 3Institute of Entrepreneurial Bio Convergence, Seoul National University, 4Seoul National University Hospital Biomedical Research Institute, Seoul National University Hospital We describe the detailed protocol for design, simulation, wet-lab experiments, and analysis for a reconfigurable DNA accordion rack of 6 by 6 meshes. Medicine Surgical Labyrinthectomy of the Rat to Study the Vestibular System Mun Young Chang1, Moo Kyun Park2, So Hyeon Park2, Myung-Whan Suh2, Jun Ho Lee2, Seung Ha Oh2 1Department of Otolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, 2Department of Otolaryngology-Head and Neck Surgery, Seoul National University Hospital This protocol describes the surgical labyrinthectomy of a rat, which is a useful method for studying the vestibular system. Behavior Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain Jaewon Beom1,2, Jung Chan Lee2,3,4, Jin Chul Paeng5, Tai Ryoon Han6, Moon Suk Bang7, Byung-Mo Oh7 1Department of Rehabilitation Medicine, Chungnam National University Hospital, Daejeon, 2Department of Biomedical Engineering, Seoul National University College of Medicine, 3Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 4Department of Biomedical Engineering, Seoul National University Hospital, 5Department of Nuclear Medicine, Seoul National University Hospital, Seoul National University College of Medicine, 6Department of Rehabilitation Medicine, Gangwon Do Rehabilitation Hospital, 7Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul National University College of Medicine We applied repetitive transcranial magnetic stimulation (rTMS) to the unilateral hemisphere of rat brain, by placing a 25-mm figure-8 coil 1 cm lateral to the vertex on the biauricular line and angulating the coil by 45°. An in-house water cooling system was used for rTMS for more than 20 min. Bioengineering Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms Jaewon Beom*1,2, Sukgyu Koh*3, Hyung Seok Nam1, Wonshik Kim3, Yoonjae Kim3, Han Gil Seo4, Byung-Mo Oh4,5, Sun Gun Chung4,5, Sungwan Kim1,6 1Department of Biomedical Engineering, Seoul National University College of Medicine, 2Department of Rehabilitation Medicine, Chungnam National University Hospital, 3Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, 4Department of Rehabilitation Medicine, Seoul National University Hospital, 5Seoul National University College of Medicine, 6Institute of Medical and Biological Engineering, Seoul National University We developed a real-time mirror robot system for functional recovery of hemiplegic arms using automatic control technology, conducted a clinical study on healthy subjects, and determined tasks through feedback from rehabilitation doctors. This simple mirror robot can be applied effectively to occupational therapy in stroke patients with a hemiplegic arm. Medicine Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs Seong Joon Ahn1,2,3, Hye Kyoung Hong1, Young Mi Na1, Sang Jun Park1,2, Jeeyun Ahn1,2,4, Jaeseong Oh5, Jae Yong Chung6, Kyu Hyung Park1,2, Se Joon Woo1,2 1Department of Ophthalmology, Seoul National University Bundang Hospital, 2Department of Ophthalmology, College of Medicine, Seoul National University, 3Department of Ophthalmology, Hanyang University Hospital, 4Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, 5Department of Clinical Pharmacology, Seoul National University Hospital, 6Department of Clinical Pharmacology, Seoul National University Bundang Hospital Rabbits are widely used to study the pharmacokinetics of intraocular drugs. We describe a method for conducting pharmacokinetic studies of intraocular drugs using rabbit eyes. Environment Enhancement of the Initial Growth Rate of Agricultural Plants by Using Static Magnetic Fields Seung C. Kim1, Alex Mason2, Wooseok Im3 1College of Medicine, Yonsei University, 2Simon Fraser University, 3Biomedical Research Institute, Seoul National University Hospital The goal of this protocol is to demonstrate the acceleration of the initial growth rate of plants by applying static magnetic fields with no external energy. Neuroscience Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium Sung Wook Park1,2, Jin Hyoung Kim2, Woo Jin Park3, Jeong Hun Kim1,2,4 1Department of Biomedical Sciences, Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, 3College of Life Sciences, Gwangju Institute of Science and Technology, 4Department of Ophthalmology, Seoul National University College of Medicine Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.