Seoul National University College of Medicine 10 articles published in JoVE Bioengineering Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats In Gul Kim1, Yanru Wu2, Su A. Park3, Hana Cho1, Jung-Woog Shin2, Eun-Jae Chung1 1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, 2Department of Biomedical Engineering, Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques. Medicine Two-Dimensional X-Ray Angiography to Examine Fine Vascular Structure Using a Silicone Rubber Injection Compound Hak Chang1, Jeong Hyun Ha1, Seong Oh Park2 1Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, 2Department of Plastic and Reconstructive Surgery, Hanyang University Medical Center, Hanyang University College of Medicine This study presents a simple two-dimensional angiographic method to examine fine vascular structures using a silicone rubber injection compound and soft tissue X-ray system. Medicine Porcine As a Training Module for Head and Neck Microvascular Reconstruction Mohammad Ali Alessa*1,2, Sang Hyun Kwak*2, Young Woo Lee2, Mi-Lan Kang3, Hak-Joon Sung3,4, Soon Hyun Ahn5, Eun Chang Choi2, Won Shik Kim5 1Department of Otolaryngology, Head and Neck Surgery, King Abdullah Medical City, 2Department of Otorhinolaryngology, Yonsei University, College of Medicine, 3Severance Biomedical Science Institute, Yonsei University, College of Medicine, 4The George W.Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 5Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine Here we present a protocol for the use of the pig superior epigastric artery perforator flap as a learning module for head and neck microvascular reconstruction. Medicine Reproducible Motor Deficit Following Aortic Occlusion in a Rat Model Of Spinal Cord Ischemia Jin-Young Hwang1, Hye-Min Sohn2, Jin-Hee Kim2, Seongjoo Park2, Jin-Woo Park2, Mi-Sun Lim2, Sung-Hee Han2 1Department of Anesthesiology and Pain Medicine, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, 2Department of Anesthesiology and Pain Medicine, SNU Bundang Hospital, Seongnamsi, Gyeonggido, Republic of Korea This study demonstrates the technique to make a minimally invasive and easily reproducible model of spinal cord ischemia in rats. Various degrees of hind limb motor deficit can be produced by controlling the aortic occlusion time. Cancer Research Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles Ji-Eun Chang1, Hyun-Jong Cho2, Sanghoon Jheon1,3 1Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital, 2College of Pharmacy, Kangwon National University, 3Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine Photodynamic therapy (PDT) is an alternative choice for lung cancer treatment. To increase the therapeutic effect of PDT, lung cancer-targeted nanoparticles combined with chemotherapy were developed. Both in vitro and in vivo anticancer efficacies of PDT with prepared nanoparticles were evaluated. Behavior Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain Jaewon Beom1,2, Jung Chan Lee2,3,4, Jin Chul Paeng5, Tai Ryoon Han6, Moon Suk Bang7, Byung-Mo Oh7 1Department of Rehabilitation Medicine, Chungnam National University Hospital, Daejeon, 2Department of Biomedical Engineering, Seoul National University College of Medicine, 3Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 4Department of Biomedical Engineering, Seoul National University Hospital, 5Department of Nuclear Medicine, Seoul National University Hospital, Seoul National University College of Medicine, 6Department of Rehabilitation Medicine, Gangwon Do Rehabilitation Hospital, 7Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul National University College of Medicine We applied repetitive transcranial magnetic stimulation (rTMS) to the unilateral hemisphere of rat brain, by placing a 25-mm figure-8 coil 1 cm lateral to the vertex on the biauricular line and angulating the coil by 45°. An in-house water cooling system was used for rTMS for more than 20 min. Bioengineering Robotic Mirror Therapy System for Functional Recovery of Hemiplegic Arms Jaewon Beom*1,2, Sukgyu Koh*3, Hyung Seok Nam1, Wonshik Kim3, Yoonjae Kim3, Han Gil Seo4, Byung-Mo Oh4,5, Sun Gun Chung4,5, Sungwan Kim1,6 1Department of Biomedical Engineering, Seoul National University College of Medicine, 2Department of Rehabilitation Medicine, Chungnam National University Hospital, 3Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, 4Department of Rehabilitation Medicine, Seoul National University Hospital, 5Seoul National University College of Medicine, 6Institute of Medical and Biological Engineering, Seoul National University We developed a real-time mirror robot system for functional recovery of hemiplegic arms using automatic control technology, conducted a clinical study on healthy subjects, and determined tasks through feedback from rehabilitation doctors. This simple mirror robot can be applied effectively to occupational therapy in stroke patients with a hemiplegic arm. Biology Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling Zai Hao Zhao1, Chun Li Jin1, Ji Hyun Jang1, Yu Na Wu1, Sung Joon Kim1, Hong Hua Jin2, Lan Cui2, Yin Hua Zhang1,2,3 1Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute, Seoul National University College of Medicine, 2Yan Bian University Hospital, 3Institute of Cardiovascular Sciences, University of Manchester This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts. Medicine Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs Seong Joon Ahn1,2,3, Hye Kyoung Hong1, Young Mi Na1, Sang Jun Park1,2, Jeeyun Ahn1,2,4, Jaeseong Oh5, Jae Yong Chung6, Kyu Hyung Park1,2, Se Joon Woo1,2 1Department of Ophthalmology, Seoul National University Bundang Hospital, 2Department of Ophthalmology, College of Medicine, Seoul National University, 3Department of Ophthalmology, Hanyang University Hospital, 4Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, 5Department of Clinical Pharmacology, Seoul National University Hospital, 6Department of Clinical Pharmacology, Seoul National University Bundang Hospital Rabbits are widely used to study the pharmacokinetics of intraocular drugs. We describe a method for conducting pharmacokinetic studies of intraocular drugs using rabbit eyes. Neuroscience Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium Sung Wook Park1,2, Jin Hyoung Kim2, Woo Jin Park3, Jeong Hun Kim1,2,4 1Department of Biomedical Sciences, Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute, Seoul National University Hospital, 3College of Life Sciences, Gwangju Institute of Science and Technology, 4Department of Ophthalmology, Seoul National University College of Medicine Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.
