Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Age 1 article published in JoVE Bioengineering Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts Aleksandra Taran*1,2, Lilia Belikova (Shuvalova)*2,3, Svetlana Lavrushkina1,2, Alexandra Bogomazova3,4, Maria Lagarkova3,4, Irina Alieva1,3 1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington's disease patients.
