National University Health System 3 articles published in JoVE Immunology and Infection Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection Zhe Zhang Ryan Lew*1, Jing Liu*1, Hsiao Hui Ong1, Vivian Jiayi Tan2, Annika Luukkainen3, Yew Kwang Ong1,4, Mark Thong1,4, Kia Joo Puan5, Vincent Tak Kwong Chow2,6, Kai Sen Tan1,2,6, De Yun Wang1,6 1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses. Immunology and Infection Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma Guo Hong*1,2, Shuangyi Fan*3, The Phyu3, Priyanka Maheshwari2, Michal Marek Hoppe2, Hoang Mai Phuong2, Sanjay de Mel4, Michelle Poon4, Siok-Bian Ng2,3, Anand D. Jeyasekharan2,4 1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections. Medicine Using Retinal Imaging to Study Dementia Victor T.T. Chan1, Tiffany H.K. Tso1, Fangyao Tang1, Clement Tham1, Vincent Mok2,3,4, Christopher Chen5,6, Tien Y. Wong7,8, Carol Y. Cheung1 1Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, 2Department of Medicine & Therapeutics, The Chinese University of Hong Kong, 3Therese Pei Fong Chow Research Centre for Prevention of Dementia, The Chinese University of Hong Kong, 4Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, 5Memory Aging and Cognition Centre, National University Health System, 6Department of Pharmacology, National University of Singapore, 7Singapore Eye Research Institute, Singapore National Eye Centre, 8Duke-NUS Medical School, National University of Singapore The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.
