Kasetsart University 4 articles published in JoVE Biology Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs Mohammad Asmat-Ullah1, Rusydi Rozaimi1, Hanafiah Fazhan1,2,3, Alexander Chong Shu-Chien2,4, Youji Wang5, Khor Waiho1,2,3 1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 2Centre for Chemical Biology, Universiti Sains Malaysia, 3Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 4School of Biological Sciences, Universiti Sains Malaysia, 5International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate. Bioengineering Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering Sougata Ghosh1,2,3, Tanay Bhagwat3, Rohini Kitture4, Sirikanjana Thongmee1, Thomas J. Webster3,5,6,7 1Department of Physics, Faculty of Science, Kasetsart University, 2Department of Microbiology, School of Science, RK. University, 3Department of Chemical Engineering, Northeastern University, 4Defence Institute of Advanced Technology Campus, Navyukti Innovations Pvt. Ltd., 5Department of Biomedical Engineering, Hebei University of Technology, 6Department of Chemistry, Saveetha School of Engineering, Institute of Medical and Technical Science, Saveetha University, 7UFPI - Universidade Federal do Piauí Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration. Immunology and Infection Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus Theerawut Phusantisampan1, Pattarasuda Rawiwan2,3, Sri Rajiv Kumar Roy2, Malinee Sriariyanun4, Win Surachetpong2,3 1 We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries. Environment Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR Pamela Nicholson1, Pattarasuda Rawiwan2, Win Surachetpong2 1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.
