National Sun Yat-sen University 6 articles published in JoVE Biology Effective Techniques for the Feeding and Ex Situ Culture of a Brooding Scleractinian Coral, Pocillopora acuta Kwok-Wai Lam1, Crystal J. McRae1, Zhao-Ting Liu2, Xuan-Ci Zhang1, Tung-Yung Fan1,2 1Department of Planning and Research, National Museum of Marine Biology & Aquarium, 2Department of Marine Biotechnology and Resources, National Sun Yat-sen University Climate change is impacting coral reef ecosystems globally. Corals sourced from ex situ aquaculture systems can help support restoration and research efforts. Herein, feeding and coral culture techniques that can be used to promote the long-term maintenance of brooding scleractinian corals ex situ are outlined. Biology Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique Ke-Rui Chou1, HsinYuan Tsai1,2 1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, 2Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously. Immunology and Infection Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining Liang-Chun Wang1, Jacob Wagner2, Annabelle Capino2, Elizabeth Nesbit2, Wenxia Song2, Daniel C. Stein2 1Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, 2Department of Cell Biology and Molecular Genetics, University of Maryland A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms. Medicine Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats Hsin-Ting Lin1,2, Yi-Lin Shiou1, Wun-Jyun Jhuang1, Hsiang-Chun Lee1,3,4,5,6 1Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 2Hematology and Oncology, China Medical University Hospital, 3Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Center for Lipid Biosciences, Kaohsiung Medical University Hospital, 5Lipid Science and Aging Research Center, Kaohsiung Medical University, 6Institute/Center of Medical Science and Technology, National Sun Yat-sen University Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine. Environment Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues Wen-Ta Li1, Bang-Yeh Liou1, Wei-Cheng Yang2, Meng-Hsien Chen3, Hui-Wen Chang1,2, Hue-Ying Chiou4, Victor Fei Pang1,2, Chian-Ren Jeng1,2 1Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, 2School of Veterinary Medicine, National Taiwan University, 3Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 4Graduate Institute of Veterinary Pathobiology, National Chung Hsing University A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues. Environment Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies Kuo-Tung Jiann*1, Liang-Saw Wen*2,3, Peter H. Santschi4 1Department of Oceanography, National Sun Yat-sen University, 2Institute of Oceanography, National Taiwan University, 3Taiwan Ocean Research Institute, National Research Laboratories, 4Department of Oceanography and Marine Sciences, Texas A&M University at Galveston Special care using "clean techniques" is required to properly collect and process water samples for trace metal studies in aquatic environments. A protocol for sampling, processing, and analytical procedures with the aim of obtaining reliable environmental monitoring data and results with high sensitivity for detailed trace metal studies is presented.
