Taichung Veterans General Hospital 2 articles published in JoVE Bioengineering Fabricating Cotton Analytical Devices Shang-Chi Lin*1, Min-Yen Hsu*2, Chen-Meng Kuan1, Fan-Gang Tseng3, Chao-Min Cheng4 1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Department of Ophthalmology, Taichung Veterans General Hospital, 3Department of Engineering and System Science, National Tsing Hua University, 4Institute of Biomedical Engineering, National Tsing Hua University To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen. Bioengineering Paper-based Devices for Isolation and Characterization of Extracellular Vesicles Chihchen Chen1, Bo-Ren Lin1, Min-Yen Hsu2, Chao-Min Cheng1 1Institute of Nanoengineering and Microsystems, National Tsing Hua University, 2Taichung Veterans General Hospital This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.