Jahangirnagar University 2 articles published in JoVE Biochemistry A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis Megumi Tsuchiya*1, M. Rezaul Karim*2, Taro Matsumoto3, Hidesato Ogawa1, Hiroaki Taniguchi3,4,5 1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences, Doshisha University We have established a method for the purification of coregulatory interaction proteins using the LC-MS/MS system. Developmental Biology Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies M. Rezaul Karim1, Tomohiro Haruta2, Taro Matsumoto3, Hiroki Oda2, Hiroaki Taniguchi3,4,5 1Biotechnology and Genetic Engineering Department, Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Graduate School of Life and Medical Sciences, Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.