2 articles published in JoVE
Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure Honghui Mao*1,2, Xiuchao Wang*1,3, Wen Chen4, FengYu Liu5, You Wan5, Sanjue Hu1, Junling Xing1,6 1Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, 2Department of Toxicology, School of Public Health, ShanXi Medical University, 3Department of Psychology, Fourth Military Medical University, 4Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, 5Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, 6Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain.
Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method Han-peng Xu1,2, Lin Gou3,4, Hong-Wei Dong3 1Department of Neurosurgery, Cedars Sinai Medical Center, UCLA, 2Basic Medicine School, Fourth Military Medical University, 3Department of Neurology, David Geffen School of Medicine, UCLA, 4Aerospace Medicine School, Fourth Military Medical Univeristy In this protocol, we described a new method to study the influence of glial cell heterogeneity on axon growth with an in vitro co-culture system. Rat cortical glial cells were cultured to confluence and cocultured with highly purified rat dorsal root ganglia neurons. Different glial cell influence on neurons adhesion and axon growth was compared directly in the same culture. This method provides a new way to directly study the glial cell heterogeneity influence on neuron adhesion and axon growth.