Southeast University (China)

10 articles published in JoVE

• Engineering

  
  Determination of Aggregate Surface Morphology at the Interfacial Transition Zone (ITZ) Kai Lyu^1,2, Wei She^{1,2 1}School of Materials Science and Engineering, Southeast University, ²State Key Laboratory of High Performance of Civil Engineering Materials Hereby, we proposed a protocol to illustrate the effect of aggregate surface morphology on the ITZ microstructure. The SEM-BSE image were quantitatively analyzed to obtain ITZ's porosity gradient via digital image processing and a K-means clustering algorithm was further employed to establish a relationship between porosity gradient and surface roughness.

• Neuroscience

  
  Cochlear Surface Preparation in the Adult Mouse Qiao-Jun Fang^1,2, Fan Wu¹, Renjie Chai², Su-Hua Sha^{1 1}Department of Pathology and Laboratory Medicine, Medical University of South Carolina, ²MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.

• Biochemistry

  
  Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy Xu Li¹, Liang Ma¹, Xiaolin Lu^{1 1}State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University Being comprehensively utilized, sum frequency generation (SFG) vibrational spectroscopy can help to reveal chain conformational order and secondary structural change happening at polymer and biomacromolecule interfaces.

• Medicine

  
  Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo Na Jia*¹, Haoan Wu*², Juanli Duan¹, Chunxue Wei¹, Kaixuan Wang², Yu Zhang ², Xiaohua Mao^{1 1}Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine, Southeast University, ²State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering, Southeast University We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

• Neuroscience

  
  Microstate and Omega Complexity Analyses of the Resting-state Electroencephalography Fei Gao¹, Huibin Jia², Yi Feng^{1 1} This article describes the protocol underlying electroencephalography (EEG) microstate analysis and omega complexity analysis, which are two reference-free EEG measures and highly valuable to explore the neural mechanisms of brain disorders.

• Chemistry

  
  A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites Li Xie¹, Liqin Chen³, Pan Gu⁴, Lanlan Wei¹, Xuejun Kang^{2 1}School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, ²Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, ³School of Public Health, Tianjin Medical University, ⁴British Columbia Academy, Nanjing Foreign Language School We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.

• Engineering

  
  Detecting the Water-soluble Chloride Distribution of Cement Paste in a High-precision Way Honglei Chang^1,2, Song Mu^{2 1}School of Materials Science and Engineering, Southeast University, ²State Key Laboratory of High Performance Civil Engineering Materials, Jiangsu Research Institute of Building Science A protocol for obtaining a water-soluble chloride profile by using a high precision milling method is presented.

• Biochemistry

  
  Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation Pan Liu¹, Tomokazu Souma¹, Andrew Zu-Sern Wei¹, Xueying Xie³, Xunrong Luo², Jing Jin^{1 1}Division of Nephrology and Hypertension, and the Center for Kidney Research and Therapeutics at the Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, ²Surgery-Organ Transplantation, Northwestern University Feinberg School of Medicine, ³School of Biological Sciences and Medical Engineering, Southeast University Mismatches in human leukocyte antigen (HLA) sequences between organ donor and recipient pairs are the major cause of antibody-mediated rejection in organ transplantation. Here we present the use of custom antigen arrays that are based on individual donors' HLA sequences to probe anti-donor HLA alloantibodies in organ recipients.

• Neuroscience

  
  The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds Michelle K. Leach¹, Zhang-Qi Feng², Caitlyn C. Gertz³, Samuel J. Tuck³, Tara M. Regan³, Youssef Naim³, Andrea M. Vincent³, Joseph M. Corey^{1,3,4 1}Department of Biomedical Engineering, University of Michigan, ²State Key Laboratory of Bioelectronics, Southeast University, ³Department of Neurology, University of Michigan, ⁴Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.

• Bioengineering

  
  Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters Michelle K. Leach¹, Zhang-Qi Feng^1,2, Samuel J. Tuck³, Joseph M. Corey^{1,3,4 1}Department of Biomedical Engineering, University of Michigan, ²State Key Laboratory of Bioelectronics, Southeast University, ³Department of Neurology, University of Michigan, ⁴Geriatrics Research, Education and Clinical Center, Veterans Affairs Ann Arbor Healthcare Center Electrospinning techniques can create a variety of nanofibrous scaffolds for tissue engineering or other applications. We describe here a procedure to optimize the parameters of the electrospinning solution and apparatus to obtain fibers with the desired morphology and alignment. Common problems and troubleshooting techniques are also presented.