2 articles published in JoVE
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC) Zach Hensel1, Xiaona Fang1,2,3, Jie Xiao1 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 2Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 3Department of Physics, Jilin University We describe a fluorescence microscopy method, Co-Translational Activation by Cleavage (CoTrAC), to image the production of protein molecules in live cells with single-molecule precision without perturbing the protein's functionality. This method has been used to follow the stochastic expression dynamics of a transcription factor, the λ repressor CI 1.
Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants Yan Zhang1,2, Kyle McCulloch2, Jarema Malicki2 1The Second Teaching Hospital of Jilin University, 2Department of Ophthalmology, Harvard Medical School Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.