Sun Yat-sen University

20 articles published in JoVE

• Medicine

  
  Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy Yiming Song*¹, Peiran Li*², Ping Lyu¹, Yanshen Yu¹, Xinyu Chen¹, Chen Cui³, Ruiye Bi², Yi Fan^{1 1}State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, ²State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthognathic and TMJ Surgery, West China Hospital of Stomatology, ³Guangdong Province Key Laboratory of Stomatology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University An animal model of acquired hypoparathyroidism (HypoPT) is crucial to understanding how HypoPT affects mineral ion homeostasis and to verify the effectiveness of novel treatments. Here, a technique is presented to generate an acquired hypoparathyroidism (AHypoPT) rat model by parathyroidectomy (PTX) using carbon nanoparticles.

• Medicine

  
  Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair Ning Ma*¹, Dai Tang*², Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Chuang-xiong Liu¹, Hao-nan Huang¹, Shuang Chen¹, Tai-cheng Zhou^{1 1}Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, ² Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain.

• Medicine

  
  Indocyanine Green-Guided Intraoperative Imaging to Facilitate Video-Assisted Retroperitoneal Debridement for Treating Acute Necrotizing Pancreatitis Li Huang¹, Wei Chen¹, Jiancong Chen¹, Dong Chen¹, Kunsong Zhang¹, Jianpeng Cai¹, Hong Peng¹, Chensong Huang¹, Guangyan Zeng¹, Mingjian Ma¹, Jiahua Liang¹, Borui Xu¹, Xiaoyu Yin¹, Jiaming Lai¹, Lijian Liang^{1 1}Department of Pancreatobiliary Surgery, The First Affiliated Hospital, Sun Yat-sen University This protocol presents Indocyanine Green-guided video-assisted retroperitoneal debridement (ICG-guided VARD) for treating severe acute necrotizing pancreatitis.

• Cancer Research

  
  Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice Qibin Fu¹, Yan Zhang¹, Tuchen Huang¹, Yang Liu^{1 1}Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice.

• Biology

  
  Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells Hongzi Chen¹, Pan Huang¹, Yan Zhang^{1 1}MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells.

• Medicine

  
  Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors Dongjie Yang*^1,2, Xun Hou*¹, Wentong Lan*³, Minxuan Shen¹, Shaohua Yang⁴, Lele Zhang¹, Qinbo Cai¹, Yan Qian¹, Yuan Lin⁵, Xia Feng⁶, Yulong He^{1,2,4 1}Center for Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, ²Guangdong Provincial Key Laboratory of Digestive Cancer Research, ³Department of Endoscopy, The First Affiliated Hospital, Sun Yat-sen University, ⁴Digestive Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, ⁵Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, ⁶Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their drawbacks.

• Cancer Research

  
  Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8⁺ T Cells in Mice Lisha Wang¹, Zhiming Wang¹, Junyi Guo², Huayu Lin¹, Shuqiong Wen², Qiao Liu¹, Yiding Li³, Qing Wu¹, Leiqiong Gao¹, Xiangyu Chen¹, Luoyingzi Xie¹, Qin Tian¹, Jianfang Tang¹, Zhirong Li¹, Li Hu¹, Juan Wang⁴, Lifan Xu¹, Qizhao Huang⁵, Lilin Ye^{1 1}Institute of Immunology, Third Military Medical University, ²Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, ³Shigatse Branch, Xinqiao Hospital, Third Military Medical University, ⁴Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, ⁵Cancer Center, The General Hospital of Western Theater Command Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.

• Medicine

  
  Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells Yuanyuan Guan*¹, Bingbing Xie*¹, Xiufeng Zhong^{1 1}State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China, 510060 Here we describe an optimized retinal organoid induction system, which is suitable for various human pluripotent stem cell lines to generate retinal tissues with high reproducibility and efficiency.

• Biology

  
  Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model Yuan Ma¹, Tao Li^{1 1}State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University This protocol describes a detailed method for the preparation and immunofluorescence staining of mice retinal flat mounts and analysis. The use of fluorescein fundus angiography (FFA) for mice pups and image processing are described in detail as well.

• Biology

  
  Detecting Migration and Infiltration of Neutrophils in Mice Qingyi Lu*¹, Kai Yuan*¹, Xiaohong Li¹, Haixu Jiang¹, Guiyang Huo¹, Wenrui Jia¹, Guangrui Huang¹, Anlong Xu^{1,2 1}School of Life Sciences, Beijing University of Chinese Medicine, ²State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University Here, we present three methods to assess neutrophil migration and infiltration both in vivo and in vitro. These methods can be used to discover promising therapeutics targeting neutrophil migration.

• Biology

  
  Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry Dianlei Guo¹, Chunqiao Liu^{1 1}State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University This protocol describes a method for the isolation of the mouse eyeball with eyelid, ocular surface, anterior and posterior segments in relatively intact position.

• Cancer Research

  
  Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma Yu Zhang¹, Yupeng Yao¹, Yan Zhang^{1 1}MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS.

• Chemistry

  
  Efficient Construction of Drug-like Bispirocyclic Scaffolds Via Organocatalytic Cycloadditions of α-Imino γ-Lactones and Alkylidene Pyrazolones Yani Zhou¹, Nannan Chen², Yaping Cheng², Xiaoqing Cai^{2 1}School of Basic Medical Sciences, Lanzhou University, ²School of Pharmaceutical Sciences, Sun Yat-sen University Enantiomerically enriched bispiro[γ-butyrolactone-pyrrolidin-4,4'-pyrazolone] skeletons are asymmetrically synthesized through a simple organocatalytic 1,3-dipolar cycloaddition reaction.

• Engineering

  
  Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons Zhaolong Cao^1,2, Min Lin², Daniel Ong^{2 1}State Key Laboratory of Optoelectronic Materials and Technologies and Guangdong Province Key Laboratory of Display Material and Technology, School of Physics and Engineering, Sun Yat-sen University, ²Department of Physics, The Chinese University of Hong Kong This protocol describes the instrumentation for determining the excitation and coupling rates between light emitters and Bloch-like surface plasmon polaritons arising from periodic arrays.

• Cancer Research

  
  A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity Yanlan Wang^1,2, Jiayu Liu^1,2, Haitao Pan^1,2, Jieyu Xing^1,2, Xiaoqiong Wu^1,2, Qing Li^1,2, Zhong Wang^{1,2 1}School of Pharmaceutical Sciences, Sun Yat-Sen University, ²Center for Cellular & Structural Biology, Sun Yat-Sen University Here, we present a protocol to produce a bispecific antibody GPC3-S-Fab in Escherichia coli. The purified GPC3-S-Fab has potent cytotoxicity against GPC3 positive liver cancer cells.

• Immunology and Infection

  
  A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals Jingshu Chen*², Lei Zhong*¹, Jing Wu¹, Sui Ke², Benjamin Morpurgo³, Andrei Golovko³, Nengtai Ouyang⁴, Yuxiang Sun², Shaodong Guo², Yanan Tian^{1,2 1}Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agriculture University, ²Texas A&M University, ³Texas A&M Institute for Genomic Medicine, ⁴Sun Yat-Sen Memorial Hospital Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic chemicals.

• Cancer Research

  
  Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro Yupeng Yao¹, Weifan Lin¹, Yan Zhang^{1 1}Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions.

• Behavior

  
  Bouncing Ball with a Uniformly Varying Velocity in a Metronome Synchronization Task Yingyu Huang*¹, Li Gu*¹, Junkai Yang*¹, Xiang Wu^{1 1}Department of Psychology, Sun Yat-Sen University The purpose of this protocol is to introduce the application of a bouncing ball with a uniformly varying velocity in a metronome synchronization task.

• Environment

  
  Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS Zhiyong Lin^1,3, Xiaoming Sun^1,2,3,4, Jörn Peckmann⁵, Yang Lu^2,3, Harald Strauss⁶, Li Xu^2,3, Hongfeng Lu⁷, Barbara M.A. Teichert^{6 1}School of Earth Sciences and Engineering, Sun Yat-sen University, ²School of Marine Sciences, Sun Yat-sen University, ³Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, ⁴South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, ⁵Institut für Geologie, Universität Hamburg, ⁶Institut für Geologie und Paläontologie, Westfälische Wilhelms-Universität Münster, ⁷Guangzhou Marine Geological Survey Analyses of the sulfur isotopic composition (δ³⁴S) of pyrite from methane-bearing sediments have typically focused on bulk samples. Here, we applied secondary ion mass spectroscopy to analyze the δ³⁴S values of various pyrite generations to understand the diagenetic history of pyritization.

• Bioengineering

  
  Porous Silicon Microparticles for Delivery of siRNA Therapeutics Jianliang Shen*^1,2, Xiaoyan Wu*^1,3, Yeonju Lee¹, Joy Wolfram^1,4, Zhizhou Yang¹, Zong-Wan Mao², Mauro Ferrari^1,5, Haifa Shen^{1,6 1}Department of Nanomedicine, Houston Methodist Research Institute, ²MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, ³Pediatrics Department of Union Hospital, Huazhong University of Science and Technology, ⁴CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, ⁵Department of Medicine, Weill Cornell Medical College, ⁶Department of Cell and Developmental Biology, Weill Cornell Medical College Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon microparticles.