3 articles published in JoVE
An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity Mengrou Liu*1, Chunsen Zhu*1, Shun Bai*2, Xin Li1, Kaiqiang Fu1, Lan Ye1, Ke Zheng1 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.
Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer Zhihui Xu*1, Yiyan Song*1, Huijun Jiang2, Yan Kong3, Xiaoming Li3, Jin Chen1, Yuan Wu4 1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening Hui Jiang*1, Yicun Wang*1, Zhantao Deng*1,2,3, Jiewen Jin2,3, Jia Meng1, Shuo Chen1, Jun Wang1, Yang Qiu1, Ting Guo1, Jianning Zhao1 1Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, 2Center for Translational Medicine, Nanjing University Medical School, 3Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.