The First Affiliated Hospital of Wenzhou Medical University 6 articles published in JoVE Neuroscience Targeted Knockdown of Genes in the Choroid Plexus Yuwen Yang*1, Chenxing Qi*1, Lanxin Hu1, Cheng Zheng1, Xuhang Li1, Wu Zheng1, Yiyun Weng2, Haiyan Lin3,4 1State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, 2Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, 3Rehabilitation Medicine Center, The Second Affiliated Hospital of Wenzhou Medical University, 4Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation, Wenzhou Medical University Here, we describe a method to selectively alter gene expressions in the choroid plexus while avoiding any impact in other brain areas. Neuroscience Establishment of a Rat Model of Superior Sagittal-Sinus Occlusion via a Thread-Embolism Method Weiyan Jiang*1, Congcong Jin*2, Weiming Xu*3, Yingxian Li4, Yinghong Lin5, Shengxiang Liang6,7, Wei Wang8 1Department of Clinical Laboratory, the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 2Rehabilitation Department, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, 3Department of Neurosurgery, the Hospital of Changle, 4Pediatrics Ward 2, Shouguang Hospital of Traditional Chinese Medicine, 5Fujian University of Traditional Chinese Medicine, 6National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, 7Rehabilitation Industry Institute, Fujian University of Traditional Chinese Medicine, 8Department of Neurosurgery, the First Affiliated Hospital of Wenzhou Medical University Here, we establish a novel Sprague-Dawley (SD) rat model of superior sagittal sinus (SSS) thrombosis via a thread-embolization method, and the stability and reliability of the model were verified. Behavior Evaluation of the Cognitive Performance of Hypertensive Patients with Silent Cerebrovascular Lesions Manman Zhang1,2, Junling Gao3, Bingjiao Xie2, Henry Ka Fung Mak4, Raymond Tak Fai Cheung2,5 1Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Medicine, The University of Hong Kong, 3Centre of Buddhist Studies, The University of Hong Kong, 4Department of Diagnostic Radiology, The University of Hong Kong, 5Research Centre of Heart, Brain, Hormone & Healthy Aging, The University of Hong Kong Here, we present a protocol to assess whether various types of silent cerebrovascular lesions are differentially associated with deficits in certain cognitive domains in a cohort of 398 hypertensive elderly Chinese, using a combination of neuropsychological tests and multi-sequence 3T MRI scanning. Medicine Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy Xuehai Chen1, Rong Yu2, Ziqiang Xu3, Yan Zhang 3, Chengyang Liu4, Bicheng Chen*1, Hao Jin*3 1Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, 2Reproductive Center, The First Affiliated Hospital of Wenzhou Medical University, 3Department of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, 4Department of Surgery, Perelman School of Medicine at the University of Pennsylvania Here, a protocol involving re-arterialized rat partial liver transplantation is presented. Specifically, 70% liver was resected in vivo by using an updated technique of vessel-oriented hepatectomy. The hepatic artery was reconstructed in an end-to-side manner. The cuff technique was modified to shorten the anastomosis time of the infrahepatic vena cava. Developmental Biology In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity Shen Zhang1, Yonggen Wu1, Yimin Weng2, Zhihui Xu1, Wenmin Chen3, Dahan Zheng4, Wei Lin5, Jun Liu6, Ying Zhou1,7 1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition. Medicine Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice Chichi Xie1,3, Weiwei Wei1, Tao Zhang1, Olaf Dirsch2, Uta Dahmen1 1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Jena University Hospital, 3Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice.
