3 articles published in JoVE
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions Lina Yu*1, Wenxiu He*1, Jie Xie*1, Rui Guo*1, Juan Ni2, Xia Zhang3, Quishi Xu1, Caifeng Wang1, Qiuling Yue1, Fangfang Li1, Mengcheng Luo4, Bo Sun3, Lan Ye1, Ke Zheng1 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions.
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity Ganggang Huang*1, Zhenwei Zhong*1, Shane Miersch2,3, Sachdev S. Sidhu1,2,3, Shin-chen Hou1, Donghui Wu1 1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains Thomas Gaj*1, Jia Liu*1,2 1Departments of Chemistry and Cell and Molecular Biology, The Scripps Research Institute, 2Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University Zinc-finger domains are intrinsically cell-permeable and capable of mediating protein delivery into a broad range of mammalian cell types. Here, a detailed step-by-step protocol for implementing zinc-finger technology for intracellular protein delivery is presented.