3 articles published in JoVE
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade Zhiping Zhang1, Shuhang Fan1, Zhiqiang Chen1, Yanzhi He1, Mengfei Huang1, Li Ding1, Yanyan Zhang5, Lin Chen1, Xinyue Zhang1,2,3,4 1College of Bioscience and Biotechnology, Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center, Yangzhou University The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses Yuyang Wang*1, Weidi Xu*1,2, Huancheng Guo1, Wenjie Gong1, Biao He1, Zhongzhong Tu1, Changchun Tu1,3, Ye Feng1 1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.
Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model Shouming Zhang1,2,3, Xueping Gao1,2,3, Genhui Xiao1,2,3,4, Chengping Lu1,2,3, Huochun Yao1,2,3, Hongjie Fan1,2,3,5, Zongfu Wu1,2,3 1College of Veterinary Medicine, Nanjing Agricultural University, 2Key Lab of Animal Bacteriology, Ministry of Agriculture, 3OIE Reference Lab for Swine Streptococcosis, 4School of Medicine, Tsinghua University, 5Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis.