Zhejiang Agricultural and Forestry University 2 articles published in JoVE Biology Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus Xiaofang Liu1, Xiang Zhou1, Lifeng Zhou1, Jiafu Hu1, Kai Guo1 1College of Forestry and Biotechnology, Zhejiang Agricultural & Forestry University Here, we introduce a detailed soaking method of RNA interference in Bursaphelenchus xylophilus to facilitate the study of gene functions. Biology Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus Najie Zhu*1, Liqun Bai*1, Stefan Schütz2, Baojun Liu3, Zhenyu Liu3, Xingyao Zhang4, Hongshi Yu1, Jiafu Hu1,5 1Department of Forest Protection, Zhejiang Agricultural & Forestry University, 2Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, 3College of Plant Protection, Shandong Agricultural University, 4Institute of Forest Protection, Chinese Academy of Forestry, 5Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.
