2 articles published in JoVE
Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography Yongyang Huang1, Jinyun Zou1, Mudabbir Badar1, Junchao Liu1, Wentao Shi5, Shunqiang Wang2, Qiongyu Guo3, Xiaofang Wang1, Sarah Kessel4, Leo Li-Ying Chan4, Peter Li4, Yaling Liu2,5, Jean Qiu4, Chao Zhou1,5,6 1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM) Yilin Wang1, Pakorn Kanchanawong2,3 1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore We present a protocol for the application of interferometric PhotoActivated Localization Microscopy (iPALM), a 3-dimensional single-molecule localization super resolution microscopy method, to the imaging of the actin cytoskeleton in adherent mammalian cells. This approach allows light-based visualization of nanoscale structural features that would otherwise remain unresolved by conventional diffraction-limited optical microscopy.