Central Nodal (Anhui) Bioscience and Technology Research Center 1 article published in JoVE Bioengineering Optical Imaging of Isolated Murine Ventricular Myocytes Shuxin Han1,2, Matt Klos3, Sherry Morgenstern3, Ramiz Ahmad3, Isabella Pua3, Shreyas Suresh1, Kayla Hicks3, Eric Devaney3 1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.
