Shenzhen Bay Laboratory

2 articles published in JoVE

• Chemistry

  
  Constructing Cyclic Peptides Using an On-Tether Sulfonium Center Chunli Song*¹, Zhanfeng Hou*^1,2, Zijun Jiao*¹, Zhaodi Liu³, Chenshan Lian^1,2, Min Zhang¹, Wei Liang³, Feng Yin^1,2, Zigang Li^{1,2 1}Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, ²Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, ³Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

• Chemistry

  
  Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry Lingxiao Chaihu^1,2, Xiaopeng Yao¹, Xiang Xu^1,2, Zhongqin Zhu¹, David D. Y. Chen³, Guanbo Wang^{1 1}School of Chemistry and Materials Science, Nanjing Normal University, ²Institute for Cell Analysis, Shenzhen Bay Laboratory, ³Department of Chemistry, University of British Columbia Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.