Shenzhen Bay Laboratory
2 articles published in JoVE
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center Chunli Song*1, Zhanfeng Hou*1,2, Zijun Jiao*1, Zhaodi Liu3, Chenshan Lian1,2, Min Zhang1, Wei Liang3, Feng Yin1,2, Zigang Li1,2 1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry Lingxiao Chaihu1,2, Xiaopeng Yao1, Xiang Xu1,2, Zhongqin Zhu1, David D. Y. Chen3, Guanbo Wang1 1School of Chemistry and Materials Science, Nanjing Normal University, 2Institute for Cell Analysis, Shenzhen Bay Laboratory, 3Department of Chemistry, University of British Columbia Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.