3 articles published in JoVE
Detecting Anastasis In Vivo by CaspaseTracker Biosensor Ho Man Tang1,2, Ming Chiu Fung2, Ho Lam Tang3 1Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, 2School of Life Sciences, Chinese University of Hong Kong, 3Department of Neurosurgery, Johns Hopkins University School of Medicine Anastasis is technically challenging to detect in vivo because the cells that have reversed the cell death process can be morphologically indistinguishable from normal healthy cells. Here we describe protocols for detecting and tracking cells that undergo anastasis in live animals by using our newly developed in vivo CaspaseTracker biosensor system.
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro Patrick Ming-Kuen Tang1,2, Philip Chiu-Tsun Tang2, Jeff Yat-Fai Chung2, Jessica Shuk Chun Hung2, Qing-Ming Wang2, Guang-Yu Lian2, Jingyi Sheng2, Xiao-Ru Huang2, Ka-Fai To1, Hui-Yao Lan2 1Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, 2Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics, The Chinese University of Hong Kong Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in vivo.
Using Retinal Imaging to Study Dementia Victor T.T. Chan1, Tiffany H.K. Tso1, Fangyao Tang1, Clement Tham1, Vincent Mok2,3,4, Christopher Chen5,6, Tien Y. Wong7,8, Carol Y. Cheung1 1Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, 2Department of Medicine & Therapeutics, The Chinese University of Hong Kong, 3Therese Pei Fong Chow Research Centre for Prevention of Dementia, The Chinese University of Hong Kong, 4Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, 5Memory Aging and Cognition Centre, National University Health System, 6Department of Pharmacology, National University of Singapore, 7Singapore Eye Research Institute, Singapore National Eye Centre, 8Duke-NUS Medical School, National University of Singapore The retina shares prominent similarities with the brain and thus represents a unique window to study vasculature and neuronal structure in the brain non-invasively. This protocol describes a method to study dementia using retinal imaging techniques. This method can potentially aid in diagnosis and risk assessment of dementia.