2 articles published in JoVE
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus Yuanchao Zhan1, Yaxin Liu1, Qinglu Zeng1,2,3 1Department of Ocean Science, Hong Kong University of Science and Technology, 2Division of Life Science, Hong Kong University of Science and Technology, 3HKUST Shenzhen Research Institute Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.
Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice Shan-Shan Li*1, Carman K. M. Ip*1, Matthew Y. H. Tang*2, Samuel K. H. Sy2, Susan Yung3, Tak-Mao Chan3, Mengsu Yang4, Ho Cheung Shum2, Alice S.T. Wong1 1School of Biological Sciences, University of Hong Kong, 2Department of Mechanical Engineering, University of Hong Kong, 3Department of Medicine, University of Hong Kong, 4Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong To study ovarian tumor progression in a physiologically relevant model, multicellular spheroids were cultured in a microdevice under simulated fluid flow. This dynamic 3D model emulates the intraperitoneal environment with the cellular and mechanical components where ovarian cancer metastasis occurs.