3 articles published in JoVE
A New Method for Inducing a Depression-Like Behavior in Rats Vladimir Zeldetz*1, Dmitry Natanel*2, Matthew Boyko2, Alexander Zlotnik2, Honore N. Shiyntum3, Julia Grinshpun2, Dmitry Frank2, Ruslan Kuts2, Evgeni Brotfain2, Jochanan Peiser2 1Department of Emergent Medicine, Soroka University Medical Center, Ben-Gurion University of the Negev, 2Division of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 3 This protocol describes a new model by which healthy rats could contract depression over a given time periodthrough contagion by exposure to chronic unpredictable stressed (CUS) rats.
High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis Itay Remer1, Lear Cohen1, Alberto Bilenca1,2 1Biomedical Engineering Department, Ben-Gurion University of the Negev, 2Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev We describe the construction of a rapid continuous-wave-stimulated-Brillouin-scattering (CW-SBS) spectrometer. The spectrometer employs single-frequency diode-lasers and an atomic vapor notch-filter to acquire transmission spectra of turbid/non-turbid samples with high spectral-resolution at speeds up to 100-fold faster than those of existing CW-SBS spectrometers. This improvement enables high-speed Brillouin material analysis.
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells Tom Shani1, Moshe Levy1, Adrian Israelson1,2 1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev This protocol describes a method to sensitively measure the nucleocytoplasmic transport rate within motor neuron-like NSC-34 cells by quantifying the real-time change in the nuclear import of a NLS-NES-GFP protein.