2 articles published in JoVE
Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo Gaurav Das1,2, Varsha Gupta1, Juhee Khan1, Deepshikha Mukherjee1, Surajit Ghosh1,2 1Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 2Academy of Scientific and Innovative Research (AcSIR) Presented here is a protocol for the spontaneous generation of neurospheres enriched in neural progenitor cells from high density plated neurons. During the same experiment, when neurons are plated at a lower density, the protocol also results in prolonged primary rat neuron cultures.
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA Hemant Suryawanshi*1, Mayuresh Anant Sarangdhar*1,2, Manika Vij1,2, Reema Roshan1, Vijay Pal Singh1, Munia Ganguli1, Beena Pillai1,2 1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.