2 articles published in JoVE
Recording Horizontal Saccade Performances Accurately in Neurological Patients Using Electro-oculogram Yasuo Terao1, Hideki Fukuda2, Yusuke Sugiyama3, Satomi Inomata-Terada1, Shin-ichi Tokushige4, Masashi Hamada3, Yoshikazu Ugawa5 1Department of Cell Physiology, Kyorin University, 2Segawa Memorial Neurological Clinic for Children, 3Department of Neurology, University of Tokyo, 4Department of Neurology, Kyorin University, 5Department of Neurology, Fukushima Medical University The article describes a practical method for recording horizontal eye movements with high accuracy by electro-oculogram in neurological patients, using a cup Ag-AgCl electrode with a wide plastic fringe. Stable measurement requires proper selection and fixation of electrodes, taking sufficient time for light adaptation to occur, and re-calibration as needed.
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor Shinya Urano1, Ryushi Tazawa1, Takahito Nei1, Natsuki Motoi1, Masato Watanabe2, Takenori Igarashi3, Masahiro Tomita3, Koh Nakata1 1Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine, Kyorin University, 3Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd. We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.