3 articles published in JoVE
Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera Yuichi Fukutomi1, Keiji Matsumoto1,2, Noriko Funayama1, Shigeyuki Koshikawa1,3,4 1Graduate School of Science, Kyoto University, 2Graduate School of Science, Osaka City University, 3The Hakubi Center for Advanced Research, Kyoto University, 4Graduate School of Environmental Science, Hokkaido University Protocols for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera are described. Staging and quantification of pigmentation provide a solid basis for studying developmental mechanisms of adult traits and enable interspecific comparison of trait development.
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level Satoshi Habuchi1, Takuya Yamamoto2,3, Yasuyuki Tezuka3 1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 2Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, 3Department of Organic and Polymeric Materials, Tokyo Institute of Technology A protocol for the synthesis and characterization of diffusive motion of cyclic polymers at the single molecule level is presented.
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue Chun-Chun Chen1, Kazuhiro Wada2, Erich D. Jarvis1 1Howard Hughes Medical Institute, Department of Neurobiology, Duke University, 2Department of Biological Sciences, Hokkaido University This protocol is successfully used to quantitatively detect levels and spatial patterns of mRNA expression in multiple tissue types across vertebrate species. The method can detect low abundance transcripts and allows processing of hundreds of slides simultaneously. We present this protocol using expression profiling of avian embryonic brain formation as an example.