Hamamatsu University School of Medicine 4 articles published in JoVE Cancer Research Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format Tomoaki Kahyo1, Haruhiko Sugimura1 1Department of Tumor Pathology, Hamamatsu University School of Medicine Digital polymerase chain reaction (PCR) is a useful tool for the high-sensitivity detection of single nucleotide variants and DNA copy number variants. Here, we demonstrate key considerations for measuring rare variants in the human genome using digital PCR with the chip-in-a-tube format. Medicine Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion Hiroki Tanaka1,2, Naoki Unno2, Tatsuro Yata2, Hirona Kugo3, Nobuhiro Zaima3, Takeshi Sasaki4, Tetsumei Urano1 1Department of Medical Physiology, Hamamatsu University School of Medicine, 2Division of Vascular Surgery, Second Department of Surgery, Hamamatsu University School of Medicine, 3Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, 4Department of Organ & Tissue Anatomy, Hamamatsu University School of Medicine Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans. Neuroscience Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain Hideya Kawasaki1, Isao Kosugi1, Makiko Sakao-Suzuki2, Shiori Meguro1, Yoshihiro Tsutsui3, Toshihide Iwashita1 1Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, 2First Department of Medicine, Hamamatsu University School of Medicine, 3Faculty of Health Science, Tokoha University Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization. Neuroscience Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons Satoru Yamagishi1, Gandhervin Kesavamoorthy1, Martin Bastmeyer2, Kohji Sato1 1Anatomy and Neuroscience, Hamamatsu University School of Medicine, 2Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT) Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.
