2 articles published in JoVE
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer Rika Azuma1, Kei Miyamoto2, Mami Oikawa3, Masayasu Yamada4, Masayuki Anzai1,5 1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse cloning.
Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion Hiroki Tanaka1,2, Naoki Unno2, Tatsuro Yata2, Hirona Kugo3, Nobuhiro Zaima3, Takeshi Sasaki4, Tetsumei Urano1 1Department of Medical Physiology, Hamamatsu University School of Medicine, 2Division of Vascular Surgery, Second Department of Surgery, Hamamatsu University School of Medicine, 3Department of Applied Biological Chemistry, Graduate School of Agriculture, Kindai University, 4Department of Organ & Tissue Anatomy, Hamamatsu University School of Medicine Polyurethane catheter insertion into the aortic lumen and suture ligation of the aorta induce chronic hypoxia due to hypoperfusion of the adventitial vasa vasorum. This article describes a novel animal model of abdominal aortic aneurysm (AAA) with characteristics similar to those of AAA in humans.