Tokyo University of Science 9 articles published in JoVE Engineering Electroantennography-based Bio-hybrid Odor-detecting Drone using Silkmoth Antennae for Odor Source Localization Daigo Terutsuki1, Tomoya Uchida2, Chihiro Fukui3, Yuji Sukekawa1, Yuki Okamoto4, Ryohei Kanzaki1 1Research Center for Advanced Science and Technology, The University of Tokyo, 2Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 3Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, 4Sensing System Research Center, National Institute of Advanced Industrial Science and Technology This study introduces experimental protocols for a bio-hybrid odor-detecting drone based on silkmoth antennae. The operation of an experimental electroantennogram device with silkmoth antennae is presented, in addition to the structure of a bio-hybrid drone designed for odor source localization using the spiral-surge algorithm. Developmental Biology Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye Yu-Han Yeh*1,2, Mengwen Hu*1,2, Toshinori Nakagawa3,4, Akihiko Sakashita1,2, Shosei Yoshida3,4, So Maezawa5,6, Satoshi H. Namekawa1,2 1 Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications. Engineering A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference Haruka Terashima1, Yoshiro Sato1, Satoshi Kobayashi1, Takaho Tsubakiyama1, Ryo Nozaki1, Satoshi Kubo1, Tomo Osada1, Kaoru Sanaka1 1Department of Physics, Tokyo University of Science We describe an optical system for the generation of unconditional polarization-entangled photons based on multiple quantum interference effects with a detection scheme to estimate the experimental fidelity of generated entangled photons. Engineering Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function Yuji Matsukawa1, Yuki Ide1, Kazuo Umemura1 1Department of Physics, Graduate School of Science, Tokyo University of Science Presented here is a protocol using a temperature-controlled microscope stage that allows a sample container to be mounted on a vertical microscope. Engineering Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells Yuji Matsukawa1, Shingo Shomura1, Kazuo Umemura1 1Department of Physics, Graduate School of Science, Tokyo University of Science A protocol for fabricating a device for simultaneously drying multiple optical cells is presented. Chemistry Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides Hidehisa Someya1, Taiki Itoh1, Mebae Kato1, Shin Aoki1,2 1Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2Imaging Frontier Center, Tokyo University of Science Here, we present protocols for the synthesis of disaccharide nucleosides by the regioselective O-glycosylation of ribonucleosides via a temporary protection of their 2',3'-diol moieties utilizing a cyclic boronic ester. This method applies to several unprotected nucleosides such as adenosine, guanosine, cytidine, uridine, 5-methyluridine, and 5-fluorouridine to give corresponding disaccharide nucleosides. Chemistry Preparation of Hollow Polystyrene Particles and Microcapsules by Radical Polymerization of Janus Droplets Consisting of Hydrocarbon and Fluorocarbon Oils Yutaka Takahashi1, Mari Kano1, Natsu Yanagisawa1, Yukishige Kondo1 1Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science A protocol for the fabrication of hollow polymer particles and microcapsules by radical polymerization using emulsions consisting of styrene, perfluoro-n-octane, and aqueous SDS (sodium dodecylsulfate) solution is presented. Bioengineering Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering Morito Sakuma1, Yoshikazu Kumashiro2, Masamichi Nakayama2, Nobuyuki Tanaka2, Yuji Haraguchi2, Kazuo Umemura1, Tatsuya Shimizu2, Masayuki Yamato2, Teruo Okano2 1Department of Physics, Tokyo University of Science, 2 Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized. Bioengineering Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology Xiaofeng Cui*1,2,3, Guifang Gao*2,4, Tomo Yonezawa5,6, Guohao Dai1 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Stemorgan Inc., 3Institute of Advanced Study, Technical University of Munich, 4Institute of Virology, School of Medicine, Wuhan University, 5Department of Molecular and Experimental Medicine, The Scripps Research Institute, 6Research Institute for Biomedical Sciences, Tokyo University of Science The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.
