Japan Advanced Institute of Science and Technology 2 articles published in JoVE Bioengineering A Nonsequencing Approach for the Rapid Detection of RNA Editing Ruchika 1, Toshifumi Tshukahara1, Manish Biyani1,2 1Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, 2BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing. Bioengineering Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface Kosuke Okeyoshi1, Kensuke Osada2, Maiko K. Okajima1, Tatsuo Kaneko1 1Japan Advanced Institute of Science and Technology, 2Department of Bioengineering, School of Engineering, The University of Tokyo A method for the drying-induced self-integration of megamolecular biopolymers on the air-liquid crystalline interface is provided here. This methodology will be useful not only for understanding the macroscopic potentials of biopolymers, but also as an evaluation method for soft materials in biomedical and environmental fields.
