Tokoha University 1 article published in JoVE Neuroscience Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain Hideya Kawasaki1, Isao Kosugi1, Makiko Sakao-Suzuki2, Shiori Meguro1, Yoshihiro Tsutsui3, Toshihide Iwashita1 1Department of Regenerative & Infectious Pathology, Hamamatsu University School of Medicine, 2First Department of Medicine, Hamamatsu University School of Medicine, 3Faculty of Health Science, Tokoha University Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.