Osaka City University Graduate School of Medicine

5 articles published in JoVE

• Biology

  
  Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes So Goto^1,2,3, Michael Frost⁴, Christine Wildsoet^{1 1}Herbert Wertheim School Optometry and Vision Science, University of California, Berkeley, ²Department of Ophthalmology, Osaka University Graduate School of Medicine, ³Department of Ophthalmology, Tokyo Medical Center, National Hospital Organization, ⁴Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.

• Immunology and Infection

  
  Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis Eunbee Park¹, Megan A. Evans⁴, Heather Doviak⁴, Keita Horitani⁴, Hayato Ogawa⁴, Yoshimitsu Yura⁴, Ying Wang², Soichi Sano^3,4, Kenneth Walsh^{1,4 1}Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, ²Department of Cardiology, Xinqiao Hospital, Army Medical University, ³Department of Cardiology, Osaka City University Graduate School of Medicine, ⁴Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models.

• Neuroscience

  
  Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI Hui Ming Khoo¹, Yuya Fujita¹, Naoki Tani¹, Tetsuya Shimokawa², Natalja Zazubovits³, Satoru Oshino¹, Jean Gotman³, Haruhiko Kishima^{1 1}Department of Neurosurgery, Osaka University Graduate School of Medicine, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³Montreal Neurological Institute and Hospital, McGill University This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products.

• Medicine

  
  Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood Yujiro Naito¹, Akio Osa¹, Kentaro Masuhiro¹, Takashi Hirai¹, Shohei Koyama¹, Atsushi Kumanogoh^{1 1}Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine We developed a simple flow cytometry assay for evaluating the binding of PD-1–blocking antibodies to T cells, requiring only a drop of peripheral blood from cancer patients.

• Cancer Research

  
  Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice Ken Kageyama^1,3, Shinji Ozaki^2,3, Takami Sato^{3 1}Department of Diagnostic and Interventional Radiology, Osaka City University Graduate School of Medicine, ²Department of Surgery, National Hospital Organization, Kure Medical Cancer Center, ³Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University Orthotopic human liver metastatic uveal melanoma xenograft mouse models were created using surgical orthotopic implantation techniques with patient-derived tumor chunk and needle injection techniques with cultured human uveal melanoma cell lines.