3 articles published in JoVE
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos Jae H. Lee1,2, Yong II Cho5, Sung S. Choi4, Hae-Won Kim1,2, Churl K. Min3, Sang J. Lee5 1Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, 2Insititute of Tissue Regeneration Engineering, Dankook University, 3Department of Biological Sciences, Ajou University, 4Department of Pharmacy, Sahmyook University, 5Department of Animal Biotechnology, Sahmyook University The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.
Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy Dexter D'Sa1, Hak-Kim Chan1, Hae-Won Kim2, Wojciech Chrzanowski1,2 1Faculty of Pharmacy, University of Sydney, 2Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University Colloidal probe nanoscopy can be used within a variety of fields to gain insight into the physical stability and coagulation kinetics of colloidal systems and aid in drug discovery and formulation sciences using biological systems. The method described within provides a quantitative and qualitative means to study such systems.
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer Rebecca E. Nakles1, Sarah L. Millman1, M. Carla Cabrera1, Peter Johnson1,2, Susette Mueller1,2, Philipp S. Hoppe3, Timm Schroeder3, Priscilla A. Furth1,2,4,5 1Department of Oncology, Georgetown University, 2Lombardi Comprehensive Cancer Center, Georgetown University, 3Stem Cell Dynamics, Helmholtz Zentrum München - German Research Center for Environmental Health, 4Department of Medicine, Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science, Dankook University Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.