3 articles published in JoVE
A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes Youkyeong Gloria Byun1, Won-Suk Chung1 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.
Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev1,2, Jinho Chung1, Dajung Jung1,3, Farnaz Sharif1,2, Ju-Young Lee1,2, Soyoun Kim1, Sebastien Royer1,2 1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.
Experimental Implementation of a New Composite Fabrication Method: Exposing Bare Fibers on the Composite Surface by the Soft Layer Method Dongyoung Lee1, Dai Gil Lee1, Jun Woo Lim2 1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 2LANL-CBNU Engineering Institute Korea, Chonbuk National University A protocol to expose bare fibers on the composite surface by eliminating resin rich area is presented. The fibers are exposed during fabrication of the composites, not by the post surface treatment. The exposed carbon composites exhibit high electrical conductivity in the through-thickness direction and high mechanical property.