3 articles published in JoVE
Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte Healin Im*1, Seok Hwan Jeong*1, Dong Hyuk Park2, Sunkook Kim1, Young Ki Hong1 1School of Advanced Materials Science and Engineering, Sungkyunkwan University, 2Department of Applied Organic Materials Engineering, Inha University A protocol for fabricating nanoporous anodic aluminum oxides via simultaneous multi-surfaces anodization followed by stair-like reverse biases detachments is presented. It can be applied repeatedly to the same aluminum substrate, exhibiting a facile, high-yield, and environmentally clean strategy.
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells Honggun Lee1, Constantin Radu1, Jeung Whan Han2, Regis Grailhe3 1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies, Institut Pasteur Korea, 2School of Pharmacy, Sungkyunkwan University, 3Technology Development Platform, Institut Pasteur Korea We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.
Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging Dasol Lee*1, Minkyung Kim*1, Sunae So*1, Inki Kim1, Gwanho Yoon1, Kyunghoon Kim2, Junsuk Rho1,3 1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2School of Mechanical Engineering, Sungkyunkwan University, 3Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) The use of a hyperlens has been regarded as a novel super-resolution imaging technique due to its advantages in real-time imaging and its simple implementation with conventional optics. Here, we present a protocol describing the fabrication and imaging applications of a spherical hyperlens.