3 articles published in JoVE
Spectral Reflectometric Microscopy on Myelinated Axons In Situ Junhwan Kwon1,2, Myunghwan Choi1,2 1Department of Biomedical Engineering, Sungkyunkwan University, 2Center for Neuroscience Imaging Research, Institute for Basic Science (IBS) Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.
Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles Byung Hyo Kim1,2, Junyoung Heo1,2, Won Chul Lee3, Jungwon Park1,2 1Center for Nanoparticle Research, Institute for Basic Science (IBS), 2School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 3Department of Mechanical Engineering, Hanyang University Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy.
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood Yang-Seok Park1, Vijaya Sunkara1, Yubin Kim1, Won Seok Lee1,2, Ja-Ryoung Han1,3, Yoon-Kyoung Cho1,4 1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3KOGAS (Korea Gas Corporation) Research Institute, 4Center for Soft and Living Matter, Institute for Basic Science (IBS) This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.